The invention relates to a method for activating human spermatozoa, applicable in the field of reproductive medicine and, in particular, in technologies for improving the functional status of male gametes prior to medical interventions related to assisted reproduction. According to the proposed method for activating human spermatozoa, powdered superhydrophobic carbon black nanoparticles with a concentration of 1000 ppm are placed in freshly separated human ejaculate. obtained by flame synthesis of rapeseed oil under controlled combustion conditions, and the resulting suspension is incubated for a period of 90-270 min at a temperature of T = 37°C, relative humidity of 98%, and CO2 content of ~5%. The advantages of the method are that very low cytotoxicity (no more than 20% deterioration in cell motility) or no cytotoxicity is observed in male gametes. In addition, the soot does not penetrate the living spermatozoa and does not stick to their surface, and activation is achieved by means of electrostatic and/or biochemical interactions with the seminal plasma. Last but not least, the synthesis of carbon soot is fast and economically feasible, and provides the possibility for additional functionalization of the material with various nanoparticles. The method for activating human spermatozoa can find wide practical application in the field of reproductive medicine in the diagnosis, selection, and subsequent improvement of the functional status of live spermatozoa in cases where intracytoplasmic injection of human gametes, classical in vitro and/or intrauterine insemination for the purposes of assisted reproduction is necessary.
